Table 2. Intraday and Interday Accuracy and Precision of LLOQ and QC Sample of HHC in Mice Plasma (Mean ± Standard Deviation (SD)).

item	nominal conc. (ng/mL)	observed conc. (ng/mL)	accuracy (%)	precision (CV%)
accuracy and precision of LLOQ sample of HHC
intra- and interday
calibration curve: y = 1.5341 × 10–3x – 1.8465 × 10–4; R2 = 0.9959
intraday (n = 3)	5.00	5.30 ± 0.30	106.04	5.66
interday (n = 9)	5.00	4.97 ± 0.66	99.47	13.19
accuracy and precision of QC sample of HHC
intraday
calibration curve: y = 1.5341 × 10–3x – 1.8465 × 10–4; R2 = 0.9959
interday
calibration curve day1: y = 1.5341 × 10–3x – 1.8465 × 10–4; R2 = 0.9959
calibration curve day2: y = 1.6891 × 10–3x – 2.2302 × 10–4; R2 = 0.9932
calibration curve day3: y = 1.7797 × 10–3x – 1.5662 × 10–3; R2 = 0.9979
intraday (n = 3)	15.00	14.80 ± 0.49	98.68	3.28
	150.00	157.60 ± 10.75	105.07	6.82
	375.00	382.78 ± 33.28	102.08	8.69
interday (n = 9)	15.00	14.39 ± 1.11	95.97	7.73
	150.00	143.38 ± 8.50	95.59	8.89
	375.00	356.74 ± 0.30	95.13	9.26