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. 2024 Aug 22;18(39):26614–26630. doi: 10.1021/acsnano.4c04814

Figure 3.

Figure 3

Targeted delivery and immune evasion performance of PM&EM/JQ1 NPs. (a) After 4h incubation with in vitro collagen, the ability of collagen to adhere to DiI NPs, PM&EM/JQ1 NPs, gray is collagen, red is encapsulated DiI fluorescent molecules, scale bar = 10 μm. (b) Fibroblasts and their collagen production stimulated by angiotensin II, green for type I collagen, blue for nuclei; scale bar = 20 μm. (c) After 4 h incubation of activated fibroblasts (stimulated with angiotensin II), cell adhesion, uptake of DiI nanoparticles, PM&EM/JQ1 NPs scale bar = 25 μm. (d) FC analysis of activated fibroblasts after 4 h incubation of DiI NPs, PM&EM/JQ1 NPs, and DiI-positive fibroblasts as a percentage of total fibroblasts (mean ± SD, n = 4 independent experiments). (e) RAW 264.7 cells were coincubated with DiI NPs and PM&EM/JQ1 NPs nanoparticles for 4 h and then subjected to laser confocal analysis, blue for nuclei, green for lysosomes, red for encapsulated DiI fluorescent molecules, scale bar = 25 μm. (f) DiI-positive macrophages as a percentage of total macrophages after coincubation with DiI-labeled nanoparticles for 4 h (mean ± SD, n = 3 independent experiments). ***p < 0.001, one-way ANOVA, Tukey’s multiple comparison test.