Fig. 2.

A Gene arrangement in ΔmcyB versus the PCC 7806 wildtype genome. In the wildtype (bottom arrangement), an IS1-family transposase is inserted before genes encoding an uncharacterized protein and a MBL-fold metallo-hydrolase protein. In ΔmcyB (top) the IS1-family transposase is absent from the intergenic region before the uncharacterized protein and MBL-fold metallohydrolase. The insertion of an IS1634 family transposase downstream of the MBL-fold metallo-hydrolase in ΔmcyB increases the length of the MBL-fold gene coding region by 80 nt residues. ​B Transcription of the genes in the order shown in Fig. 2A. Absence of the IS1-family transposase in the mutant leads to significantly increased expression (p < 0.001) of the uncharacterized protein and MBL-fold metallo-hydrolase compared to the expression of these genes in the wildtype, which has the IS1 transposase insertion