TABLE 2.

Demographic characteristics, clinical characteristics, and laboratory results of ocular syphilis patients with T. pallidum PCR, genotyping, and WGS data (n = 17)^a

Specimen ID	Location (state)	Sex	Sexual orientation	HIV status	Stage of syphilis	RPR titer	CSF-VDRL titer	Sample	polA PCR	polA Ct	23S rRNA mutation	ACDCT strain type	MLST allelic profile	T. pallidum clade	Diagnosis
OS-1	Texas	Male	MSM/W	Pos	Unknown	R	1:2	CSF	Pos	44.8	NA	14b9/g	NA	NA	Posterior uveitis, blurry vision
OS-2	Georgia	Male	MSM	Pos	Late latent	1:512	1:4	CSF	Pos	43.3	NA	NA	NA	NA	Panuveitis, partial vision loss
OS-3	Hawaii	Male	MSW	Neg	Secondary	1:256	R	CSF,blood,plasma,pharyngeal swab	Neg Neg Neg Pos	NA NA NA 32.4	NA NA NA NA	NA NA NA _b _9/_	NA NA NA NA	NA NA NA NA	Chorioretinitis, optic neuritis, blurry vision
OS-4	New York	Male	Unknown	Pos	Unknown	1:512	R	CSF,blood,serum	Pos Pos Neg	43.4 36.9 NA	NA NA NA	14_10/g 14d10/g NA	NA X3X NA	NA SS14 NA	Posterior uveitis
OS-5	Massachusetts	Male	MSM	Neg	Secondary	Unknown	1:4	CSF	Pos	38	NA	_ _ _/f	NA	NA	Retinal hypopigmentation
OS-6	Washington	Male	MSM	Neg	Secondary	1:2,048	1:2	CSF	Pos	33.5	NA	14_ _/_	NA	NA	Blurry vision
OS-8	Massachusetts	Male	MSM	Pos	Secondary	1:128	Unknown	CSFBlood	Pos Neg	35.7 NA	NA NA	NA NA	NA NA	NA NA	Posterior uveitis, blurry vision
OS-9	Massachusetts	Male	MSM	Pos	Secondary	1:256	Unknown	CSF	Pos	37.2	NA	NA	NA	NA	Panuveitis, partial vision loss, blurry vision
OS-10	North Carolina	Male	MSM	Unknown	Unknown	1:128	1:8	CSF	Pos	37.5	NA	NA	NA	NA	Panuveitis, blurry vision
OS-11	New York	Male	Unknown	Pos	Secondary	1:4	R	Vitreous fluid	Pos	33.9	NA	NA	NA	NA	Panuveitis, optic neuritis, chorioretinitis
OS-12	Massachusetts	Female	WSM	Neg	Secondary	1:2	R	CSF,vitreous fluid	Pos Pos	31.1 24.7	NA A2058G	14e9/f 14e9/f	X.1.1 19.1.1	SS14	Panuveitis
OS-13	Massachusetts	Male	MSW	Neg	Secondary	1:128	NR	CSF, blood	Pos Pos	38.3 41.3	NA NA	NA 14d_/_	NA NA	NA NA	Panuveitis
OS-14	Idaho	Female	WSM	Neg	Late latent	Unknown	R	CSF	Pos	40.8	NA	NA	NA	NA	Uveitis, chorioretinitis, loss of vision in both eyes
OS-15	New York	Male	MSM	Pos	Early latent	1:128	NR	CSF	Pos	35.7	NA	_e_/_	NA	NA	Posterior uveitis
OS-16	Massachusetts	Male	Unknown	Pos	Late latent	1:512	NR	CSF, plasma	Neg Pos	NA 34.3	NA NA	NA NA	NA NA	NA NA	Optic neuritis, papillitis, blurry vision
OS-17	Idaho	Male	MSM	Pos	Unknown	1:65,536	1:2	CSF	Pos	37.1	NA	NA	NA	NA	Panuveitis, uveitis, partial vision loss, blurry vision
OS-18	Connecticut	Male	MSM	Neg	Secondary	1:128	Unknown	Vitreous fluid	Pos	26.9	A2058G	14d9/g	6c.3.1	SS14	Chorioretinitis, posterior uveitis, vitritis

^a Abbreviations: ACDCT, augmented CDC typing; CSF, cerebrospinal fluid; Ct, cycle threshold; MLST, multilocus sequence typing; MSM, men who have sex with men; MSW, men who have sex with women; WSM, women who have sex with men; RPR, rapid plasma regain, VDRL, Venereal disease research laboratory; Pos, positive; Neg, negative; NR, non-reactive; R, reactive; NA, not amplifiable; NT, not tested; X, MLST allele not determined.

^b Denotes ACDCT typing targets that were not amplifiable.

^c Closest match for tp0136 was to allele 6 with one indel mutation.