MFS has an additive interaction with ITZ, TRB, and AMB against S. brasiliensis and S. schenckii yeast cells. The synergy score for MFS × TRB, MFS × ITZ, and MFS × AMB against Sporothrix was determined by analyzing the checkerboard assay data with SynergyFinder software. (a) S. schenckii and (b) S. brasiliensis yeasts were grown in liquid YDP pH 7.8 at 37°C in different concentrations of the selected drugs. After 4 days of incubation, the metabolic activity of the cells was assessed by the XTT reduction assay. The results are expressed as the % of metabolic activity and represent the average of three independent experiments.