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. 2024 Aug 28;12(10):e00474-24. doi: 10.1128/spectrum.00474-24

Fig 4.

Yeast cells were treated with varying concentrations of miltefosine and visualized with DIC and PI staining to indicate cell death. Bar graph on the right quantifies the percentage of PI-positive (dead) cells.

MFS causes dose-dependent death in S. brasiliensis yeast. (a) S. brasiliensis yeasts were exposed to 0, 2, 4, and 8 µg/mL of MFS for 6 h, stained with PI, and analyzed by fluorescence microscopy. (b) Quantification of PI+ yeast exposed to MFS, in which 100 yeast-like cells were counted for each condition. The results represent the average of two independent experiments. **P value < 0.001 when compared with untreated cells; ns: not significant.