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. 1985 Apr 1;227(1):163–167. doi: 10.1042/bj2270163

New insights on the cytochrome c oxidase proton pump.

M Thelen, P S O'Shea, A Azzi
PMCID: PMC1144821  PMID: 2986602

Abstract

Cytochrome c oxidase vesicles were used to show that, under appropriate experimental conditions: (1) no net deprotonation of the vesicular membrane or of the incorporated enzyme occurs during the oxidation of ferrocytochrome c; (2) the pH equilibration kinetics of a respiration-induced pH gradient across the bilayer are a simple function of the ohmic proton-conductance properties of the membrane; (3) a fairly constant stoichiometry (0.8-0.7) of the numbers of protons pumped per molecule of ferrocytochrome c oxidized, i.e. the H+/e- ratio, over a wide range of dioxygen molecules reduced (1-12) is observed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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