TABLE 1.
Cell cycle distribution of H1299 cells transiently expressing st or mutantsa
| Vector | Growth stage | % Increase in cell population for expt:
|
|||
|---|---|---|---|---|---|
| I. Fugene | II. Fugene | III. CaPO4 | IV. CaPO4 | ||
| pcDNA3 | G0/G1 | 61.8 | 66.5 | 64.6 | 61.4 |
| S | 26.1 | 27.8 | 27.6 | 25.7 | |
| G2/M | 12.0 | 5.8 | 7.8 | 12.9 | |
| st | G0/G1 | 48.5 | 50.4 | 37.5 | 33.3 |
| S | 36.2 | 39.5 | 37.9 | 37.3 | |
| G2/M | 15.3 | 10.1 | 24.7 | 29.4 | |
| st D44N | G0/G1 | 53.2 | 56.2 | 47.5 | |
| S | 38.9 | 32.6 | 34.6 | ||
| G2/M | 7.9 | 11.1 | 17.9 | ||
| st C103S | G0/G1 | 62.0 | 71.5 | 60.1 | |
| S | 28.9 | 22.7 | 28.7 | ||
| G2/M | 9.1 | 5.8 | 11.2 | ||
| st TR4 | G0/G1 | 59.2 | 70.8 | 62.0 | |
| S | 29.6 | 21.3 | 27.6 | ||
| G2/M | 11.2 | 7.9 | 10.4 | ||
| st C103S/TR4 | G0/G1 | 62.6 | 77.3 | 65.5 | |
| S | 26.0 | 16.4 | 25.9 | ||
| G2/M | 11.4 | 6.3 | 8.6 | ||
a
H1299 cells were transiently transfected with an expression vector for st, or mutant st, together with a cell surface marker, CD20. After 72 h, the cell cycle distribution of the st-expressing population was assessed using FACS and gating on the CD20-positive cells. This table shows cell cycle distributions from four independent experiments which produced consistent results. Experiments I and II used Fugene-6 for the transfection protocol, while experiments III and IV employed calcium phosphate.