FIG. 1.

Schematic representation of sequential proteolytic processing of HIV-1 Gag (G) and Gag-Pol (GP) precursor proteins by viral PR and the initial proteolysis rate of the primary (1°), secondary (2°), and tertiary (3°) cleavage sites of Gag (slower) adapted from Pettit et al. (42, 43). The scissors symbol represents corresponding locations cut by PR in HIV-1. In this study, constructs containing mutations that do not allow the processing of the primary (p2/NC), secondary (MA/CA and p1/p6), and tertiary (CA/p2) cleavage sites, from two different sources, were analyzed for their protein profiles and genomic RNA dimer maturation.