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Impact of mutations within the primary, secondary, and late-proteolytic processing cleavage sites of HIV-1 on the protein composition of purified virions. Viral proteins were semiquantified by Western blot analysis. Purified virions were resolved by using 10% Tris-glycine SDS-PAGE (i) and by 16.5% Tris-Tricine SDS-PAGE (ii). Resolved proteins were probed with anti-p24 MAb or anti-p7 polyclonal antibody with specific activity to NC protein, as described in Materials and Methods.
