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. 2024 Aug 21;12(10):e00829-24. doi: 10.1128/spectrum.00829-24

Fig 2.

Western blots depict A2B1 and β-actin in WT and A2B1−/− THP-1 and MEF cells. SFTSV NP in WT and A2B1−/− THP-1 cells, and in BMDMs with or without Hnrnpa2b1 deletion. Bar graphs depict SFTSV VP mRNA fold changes.

HnRNP A2B1 upregulates SFTSV replication. (A) Knockout of hnRNP A2B1 in THP-1 cells and MEF cells was confirmed with Western blot. (B) BMDM cells were isolated from A2B1fl/fl and A2B1fl/flLyz2-Cre–/– mice. WT and A2B1-/- THP-1 and BMDM cells were infected with SFTSV at an MOI of 10 for 48 h, and SFTSV NP protein levels were analyzed with Western blot. (C) WT and A2B1-/- THP-1 and BMDM cells were infected with SFTSV at an MOI of 10 for 48 h, and SFTSV NP mRNA levels were analyzed with RT-qPCR. (D) WT and A2B1-/- MEF cells were infected with SFTSV for the indicated time points. SFTSV titers in cell culture supernatant were measured with immunofluorescence assay. (E) MEF cells were transfected with HA-tagged A2B1 for 24 h and then were infected with SFTSV at an MOI of 10 for 48 h. SFTSV NP protein levels were analyzed with Western blot. (F) WT and A2B1-/- MEF cells were transfected with HA-tagged A2B1 for 24 h, and then were infected with SFTSV at an MOI of 10 for 48 h. and SFTSV NP mRNA levels were analyzed with RT-qPCR. (G) WT and A2B1-/- MEF cells were transfected with HA-tagged A2B1 for 24 h, A2B1 protein levels were analyzed with Western blot. Data were obtained from three independent experiments (n = 3). **P < 0.01, ***P < 0.001, ****P < 0.0001, ns, not significant.