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. 2024 Aug 21;12(10):e00829-24. doi: 10.1128/spectrum.00829-24

Fig 3.

Western blots depict A2B1, LaminB1, and Akt in the nucleus and cytoplasm after infection. Bar graphs display A2B1/LaminB1 and A2B1/Akt ratios. Other bar graphs depict SeV, VSV, EV71, and ZIKV mRNA fold changes in mock and infected cells.

HnRNP A2B1 undergoes nucleocytoplasmic translocation after infection with other RNA viruses. (A–D) THP-1 cells were infected with SeV, VSV, EV71, or ZIKV at an MOI of 10 at the indicated time for each virus. Nuclear and cytoplasmic proteins were separated. A2B1, Lamin B1, and Akt protein levels were analyzed with Western blot. Lamin B1 and Akt were nuclear and cytoplasmic index proteins, respectively. Nuclear and cytoplasmic Western blot data were semi-quantified and normalized against LaminB1 and Akt protein loading control, respectively. (E) THP-1 cells were infected with SeV, VSV, EV71, and ZIKV at an MOI of 10 for 48 h, 24 h, 12 h, and 24 h. SeV, VSV, EV71, and ZIKV mRNA levels were analyzed with RT-qPCR, respectively.