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. 2024 Aug 21;12(10):e00829-24. doi: 10.1128/spectrum.00829-24

Fig 6.

Experiments depict no significant difference in SFTSV NP mRNA binding or internalization between CRISPR V2 and A2B1-/- cells across multiple viruses. A2B1 immunoprecipitation demonstrates association with SFTSV RNA segments.

HnRNP A2B1 interacts with the 5′ UTR of SFTSV RNA. (A) WT and A2B1-/- MEF cells were infected with SFTSV, SeV, VSV, EV71, and ZIKV at an MOI of 10 at 4°C for 1 h, SFTSV NP, SeV, VSV, EV71, and ZIKV mRNA levels were analyzed with RT-qPCR. (B) WT and A2B1-/- MEF cells were infected with SFTSV, SeV, VSV, EV71, and ZIKV at an MOI of 10 at 4°C for 1 h, following incubation at 37°C for 2 h, SFTSV NP, SeV, VSV, EV71, and ZIKV mRNA levels were analyzed with RT-qPCR. (C) MEF cells were infected with SFTSV at an MOI of 10 for 24 h, and the interaction between S, M, or L segment RNA of SFTSV and hnRNP A2B1 was detected with RNA immunoprecipitation (RIP). (D) HEK293T cells were transfected with HA-hnRNP A2B1 plasmids for 24 h, and the interaction between 3′ or 5′ UTR of SFTSV S segment RNA and hnRNP A2B1 was detected with RNA pulldown assay. Data were obtained from three independent experiments (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.