Table 2.
Nucleotide BLASTn results of filarial and Wolbachia sequences in the present study
| Case | Locus | Amplicon Size (bp) | GenBank Accession No. | BLASTn Analysis (closest sequence) | % Query Coverage | % Identity |
|---|---|---|---|---|---|---|
| 1 | 12 S rRNA | 109 | MT135222.1 | Brugia pahangi (AP017680.1) | 100 | 96.33 |
| ITS1 | 492 | OQ780598.1 | Brugia pahangi C46Cat5 (EU419351.1) | 100 | 92.72 | |
| 2 | 12 S rRNA | 109 | MT135223.1 | Brugia pahangi 46YT MNHN (KP760318.1) | 100 | 100 |
| COI | 657 | OQ713625.1 | Brugia pahangi THAI62 (MT027204.1) | 100 | 99.54 | |
| ITS1 | 502 | OQ780599.1 | Brugia pahangi C18Cat7 (EU373645.1) | 100 | 93.02 | |
| 3 | 12 S rRNA | 109 | MT887286.1 | Brugia malayi L01 (OQ727407.1) | 100 | 100 |
| COI | 657 | OQ713626.1 | Brugia malayi D14 (MK250720.1) | 100 | 99.85 | |
| ITS1 | 511 | MT732324.1 | Brugia pahangi C14Cat7 (EU373652.1) | 100 | 98.83 | |
| 4 | 12 S rRNA | 110 | MT584737.1 | Brugia malayi 8YT MNHN (KP760317.1) | 100 | 100 |
| COI | 657 | OQ713627.1 | Brugia malayi (NC004298.1) | 100 | 100 | |
| ITS1 | 497 | OQ780600.1 | Brugia pahangi C46Cat5 (EU419351.1) | 100 | 91.80 | |
| 5 | 12 S rRNA | 113 | MW051483.1 | Dirofilaria sp. “hongkongensis” HKU1-HKU9 (KY750550.1) | 100 | 99.12 |
| COI | 657 | OQ713628.1 | Dirofilaria sp. “hongkongensis” (MN564742.1) | 100 | 99.85 | |
| ITS1 * | 608 | OQ780601.1 | Dirofilaria repens (AB973229.1) | 100 | 96.22 | |
| Wolbachia 16S rRNA | 415 | OQ780644.1 | Wolbachia sp. in Ctenocephalides felis wCfeF (CP116767.1) | 99 | 99.27 | |
| 6 | 12 S rRNA | 105 | OK429322.1 | Pelecitus copsychi 268-5 MNHN (OK480977.1) | 99 | 98.08 |
| COI | 657 | OQ713629.1 | Pelecitus copsychi 1684 MNHN (OK480041.1) | 92 | 90.91 | |
| ITS1 * | 577 | OQ780602.1 | Dirofilaria repens (AB973229.1) | 70 | 98.76 | |
| Mansonella perstans (MN432520.1) | 69 | 98.02 | ||||
| 7 | 12 S rRNA | 113 | OQ732706.1 | Dirofilaria sp. “hongkongensis” HKU1-HKU9 (KY750550.1) | 100 | 100 |
| COI | 669 | OQ713630.2 | Dirofilaria sp. “hongkongensis” mitochondrion genome (NC_031365.1) | 100 | 99.85 | |
| ITS1 | 505 | OQ780603.1 | Dirofilaria sp. CRS-2021 isolate 20115 (MZ736411.1) | 96 | 98.98 | |
| Dirofilaria sp. “hongkongensis” HKU2 (JX290195.1) | 74 | 98.14 | ||||
| Wolbachia 16S rRNA | 428 | OQ780645.1 | Wolbachia sp. in Dirofilaria repens (MK050782.1), Dirofilaria immitis (AF487892.1) and Onchocerca sp. (JX075217.1) | 99–100 | 98.83 |
*
The ITS1 sequences of cases 5 and 6 were amplified using Dirofilaria ITS1-specific primers.