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. 2024 Aug 6;111(4):785–790. doi: 10.4269/ajtmh.24-0028

Table 2.

FLA detected by NNA culture and multiplex qPCR methods in water samples from Tenerife Island

Water Sample NNA Culture + PCR qPCR
Ac Nf Bm Vv Ac Nf Bm Vv
n % n % n % n % n % n % n % n %
Tap water (n = 19) 4 21.0 0 0 0 0 2 10.5 5 26.3 0 0 0 0 3 15.8
Stagnant water (n = 19) 2 10.5 0 0 0 0 2 10.5 12 63.2 0 0 0 0 8 42.1
Total (N = 38) 6 15.8 0 0 0 0 4 10.3 17 44.7 0 0 0 0 11 28.9
7/38 (18.4%) 18/38 (47.4%)

Ac = Acanthamoeba spp.; Bm = B. mandrillaris; FLA = free-living amoebae; Nf = N. fowleri; NNA = nonnutritive agar; qPCR = real-time quantitative polymerase chain reaction; Vv = V. vermiformis.