Fig. 6.

Impact of CD151 overexpression on junctional proteins, stress fibers, and RhoA activity. a Western blotting analysis of the indicated lysates with anti-occludin, ZO-1, and VE-cadherin antibodies Pre-OGD and at 3 h and 4 h of reoxygenation after OGD. b Western blotting analysis of the indicated lysates with anti-F-actin antibody Pre-OGD and at 3 h of reoxygenation after OGD. *p < 0.05, ** < .0.01. c Quantification of ZO-1expression level in wild-type-(WT) BMVECs, Lenti-BMVECs and Lenti-CD151 BMVECs Pre-OGD and at 3 h and 4 h of reoxygenation after OGD. d Quantification of VE-cadherin expression level in WT-BMVECs, Lenti-BMVECs and Lenti-CD151 BMVECs Pre-OGD and at 3 h and 4 h of reoxygenation after OGD. e Quantification of Occludin expression level in WT-BMVECs, Lenti-BMVECs and Lenti-CD151 BMVECs Pre-OGD and at 3 h and 4 h of reoxygenation after OGD. f Representative photograph of stained BMVECs to visualize F-actin (red) and nucleus (blue) pre OGD and at 3 h of reoxygenation after OGD. Scale bar, 50 µm. g Quantification of F-actin expression level in wild-type-(WT) BMVECs, Lenti-BMVECs and Lenti-CD151 BMVECs. Lenti-CD151 BMVECs showed less F-actin expression levels than Lenti-BMVECs at 3 h of reoxygenation after OGD. *p < 0.05. h Bar graph represents RhoA activity. RhoA activity of Lenti-CD151 BMVECs was less compared to that of Lenti-BMVECs and WT-BMVECs at 3 h of reoxygenation after OGD. *p < 0.05