Figure 5.

(A) Representative flow cytometry DAPI cell cycle histograms (bottom panel) in different CD71 expression categories (top panel). (B) Representative FACS dot plots of OCIAML2 cells transduced with the indicated overexpression vector (Dsred+) and stained for CD71. (C) Mean Fluorescence Intensity (MFI) of CD71 on sWT1 transcript expressing and non-transduced cells. Significance was determined by paired two-tailed t tests. (D) Schematic illustrating the experimental workflow of evaluating cytarabine sensitivity in CD71^high and CD71^dim expressing leukemia samples-populations for cytarabine treatment. Cells were treated with cytarabine for 24-48 hours and quantified by MTS viability assay. (E) The graph depicts higher mean ± SEM of cell viabilities of CD71^dim cells compared to CD71^high patient cells treated with cytarabine for 48 hours determined by MTS assays. (F) Representative FACS histograms depict decreased CD71 expression in both CD71^high and CD71^dim cells treated with cytarabine. (G) The graph depicts the mean (from four technical replicates) MTS absorbance of both CD71^high and CD71^dim leukemia cells culture for 48 hours in basal Stemspan medium. Statistical significances were assessed using two-tailed tests and expressed as **p < 0.01. (H) Graphs depict decreased CD71 expression in both CD71^high and CD71^dim cells treated with cytarabine from three donors. (I) The bar graphs depict real time PCR ∆∆Ct values of WT1, HOXA3, and GATA2 in sorted CD71− cells compared to CD71+ cells from 4 different AML samples. HPRT was used as the reference control. Statistical significance was determined using two-tailed Student’s t-tests (Mann-Whitney test).