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Expression of codon-optimized HPV-16 L2. 293T cells were transfected with expression constructs containing HPV-16 L2ori, HPV-11 L2ori or HPV-16 L2h. The L2 protein was subsequently detected by Western blotting in extracts of transfected cells by use of a polyclonal rabbit antiserum specific for HPV-16 and HPV-11 L2 (a) or by indirect immunofluorescence of transfected cells (b).
