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CD8+ T-cell specificity of IFN-γ responses to synthetic peptides. PBMC from donor 00BW1774 were CD8+ CD4+ depleted or enriched by using MACS CD8 MicroBeads and CD4 MicroBeads followed by Elispot assay with 20-mer Gag p24 peptide MFTALSEGATPQDLNTMLNT and 9-mer Gag p24 peptide TPQDLNTML (G180-TL9). Experiments were performed in triplicate. The extent of standard deviation is shown (error bars).
