| Method |
Sitting-drop vapor diffusion for initial crystal screening, hanging-drop vapor diffusion for crystal optimization |
| Plate type |
96-well MRC plates for initial crystal screening and 24-well plates for crystal optimization |
| Temperature (K) |
293 |
| Protein concentration (mg ml−1) |
7.5 |
| Protein buffer composition |
25 mM Tris–HCl pH 7.5, 100 mM sodium chloride, 5 mM β-mercaptoethanol |
| Composition of reservoir solution |
0.1 M sodium acetate trihydrate pH 4.6, 0.15 M ammonium acetate, 30% PEG 4000 |
| Volume and ratio drop |
2 µl; 1:1 protein:reservoir solution |
| Volume of reservoir solution |
70 µl for initial crystal screening, 1.0 ml for optimization |
