| Method |
Vapor diffusion, sitting drop |
| Plate type |
96-well plates |
| Temperature (K) |
290 |
| Protein concentration (mg ml−1) |
13.5 |
| Buffer composition of protein solution |
20 mM HEPES pH 7.0, 300 mM NaCl, 5%(v/v) glycerol, 1 mM TCEP, 0.4 mM Myr-CoA, 0.4 mM IMP-0001173 |
| Composition of reservoir solution |
0.06 M magnesium chloride hexahydrate, 0.06 M calcium chloride dihydrate, 0.1 M Tris–Bicine pH 8.5, 19.6%(v/v) PEG 500 MME, 9.8%(w/v) PEG 20 000 |
| Volume and ratio of drop |
0.4 µl, 1:1 |
| Volume of reservoir (µl) |
80 |
| Composition of cryoprotectant solution |
20%(v/v) ethylene glycol, 0.06 M magnesium chloride hexahydrate, 0.06 M calcium chloride dihydrate, 0.1 M Tris–Bicine pH 8.5, 19.6%(v/v) PEG 500 MME, 9.8%(w/v) PEG 20 000 |
