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List of primers used for real-time PCR
| Gene | Sequences of primers | Melting temperature (°C) | Product length (bp) |
|---|---|---|---|
| RPL-19 | F: TCGCCAATGCCAACTCTCGTC | 63 | 92 |
| R: AGCCCGGGAATGGACAGT | 65 | ||
| Bax | F: TGCTACAGGGTTTCATCCAG | 58 | 135 |
| R: CCAGTTCATCGCCAATTC | 57 | ||
| Bcl-2 | F: TGGAAAGCGTAGACAAGGAGATGC | 65 | 88 |
| R: CAAGGCTCTAGGTGGTCATTCAGG | 67 | ||
| PARP | F: CGCTCAAGGCTCAGAACGAG | 63 | 130 |
| R: CAGGATTGCGGACTCTCCA | 59 | ||
| caspase 3 | F: GATGTCGATGCAGCTAACC | 57 | 321 |
| R: TGTCTCAATACCGCAGTCC | 57 |
