FIG. 8.

Transcription of tk and rta genes is suppressed in stable cell lines expressing Rd2. Two cell lines, 45-5 and V-30, stably transfected with pCMVFLAG/Rd2 and the parental 293T cells (P) were infected with wild-type MHV-68 (3 PFU/cell). Total RNA from uninfected (U) or infected cells was harvested at 4 or 15 h postinfection, and 30% of each RNA sample was used for Northern blot analysis. (A) Transcription of rta is reduced in 45-5 and V-30 cells. The probe was derived from the rta gene (nt 68651 to 69378). (B) Transcription of tk is reduced in 45-5 and V-30 cells. The same membrane was stripped and rehybridized with a probe derived from the tk gene (nt 32879 to 34813). (C) The RNA loadings were examined by rehybridizing with a probe derived from the cellular GAPDH (glyceraldehyde-3-phosphate dehydrogenase) gene.