Fig. 3.
Blocking the wound calcium wave and quantifying the resulting changes in cell behaviours. (A) Spatio-temporal heatmaps of cell velocity relative to wounds for control 14.75 h APF large wounds. Blue regions indicate those cells that are migrating away from the wound and red indicates those cells migrating towards the wound. (B) Spatio-temporal heatmaps of cell shape elongation relative to wounds for control 14.75 h APF large wounds. Blue regions indicate cells elongated perpendicular to the wound and red indicates cells oriented towards the wound. (C) Heatmaps of cell division density for control 14.75 h APF large wounds. (D,D′) Still images from gCAMP movies at 0 and 26 s post-wounding of control versus TrpmRNAi wing tissues to show a blocked calcium wave around TrpmRNAi wounds. White asterisks indicate the location of the wound. (E) Quantification of the wound area over time as large wounds close. Red dashed line is the 9-10 min point where we classify the size of wounds. (F) Spatio-temporal heatmaps of cell velocity relative to wound centre for large wounds. Blue regions indicate cells that are migrating away from wounds and red indicates those migrating towards the wound. (G) Spatio-temporal heatmaps of cell shape elongation relative to wounds for large wounds. Blue regions indicate cells that are elongated perpendicular to wounds and red indicates cells oriented towards the wound. (H) Heatmaps of cell division density for large wounds. (I) Spatio-temporal heatmaps of the change in cell velocity for control versus wounds with a blocked calcium wave. Blue regions indicate slower cell migration towards the wound than controls and red regions indicate faster cell migration. (J) Spatio-temporal heatmaps of the change in cell shape elongation for control versus calcium wave-blocked wounds. Blue regions indicate that cells are more elongated along the wound margin than controls and red regions indicate that cells are more oriented towards the wound. (K) Heatmaps of the change in division density for control versus calcium wave-blocked wounds. Blue regions indicate lower cell density than controls and red regions indicate higher cell density. All heatmaps are weighted means of a cell behaviour for each wound video with the weight corresponding to the visible area of tissue in videos. n=5 control, 14.75 h APF wounds; n=4 TrpmRNAi wounds. Scale bars: 50 μm.