| Oligo | Sequence | Use |
|---|---|---|
| MS266 | /5InvddT/G*G*G*A*A*CCTCTTCTGTAACTCCTTAGC | Amplify HDR template |
| MS267 | /5InvddT/C*C*T*G*A*G*GGCAAACAAGTGAGCAGG | Amplify HDR template |
| MS269 | TCGTGCCAGCCCCCTAATCT | Validate eIF2Bα tagging |
| MS270 | CTGAACGGCGCTGCTGTAGC | Validate eIF2Bα tagging |
| MS256 | AGTGAACTCTACCATCCTGA | Validate eIF2Bβ tagging |
| MS258 | TTAGGTGGACTCCTGTGC | Validate eIF2Bβ tagging |
| MS096 | CTGGCTAACTGGCAGAACC | Validate eIF2Bδ tagging |
| MS268 | AGAAACAAAGGCAGCAGAGT | Validate eIF2Bδ tagging |
| sgMS001 | CAATCTGCTTAGGACACGTG | Target Cas9 to eIF2Bβ C-terminus |
| sgMS004 | AGAGCAGTGACCAGTGACGG | Target Cas9 to eIF2Bδ C-terminus |
| sgMS006 | GAGGACGCCATGGACGACAA | Target Cas9 to eIF2Bα N-terminus |
| HDR: homology-directed recombination. | ||
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