TABLE 1.
Generation of recTHOV, following plasmid transfection of 293T cells
| Hours after plasmid transfection | Virus titers in culture supernatant (PFU/ml) in expta:
|
||||
|---|---|---|---|---|---|
| 1 (complete) | 2 (complete) | 3 (complete) | 4 (−T7/M) | 5 (−T7/M) | |
| 24 | 0 | 0 | 0 | 0 | 0 |
| 48 | 10 | 0 | 0 | 0 | 0 |
| 72 | 1.1 × 104 | 0 | 0 | 0 | 0 |
| 96 | 1 × 106 | 0 | 90 | 0 | 1 |
| 120 | 1.5 × 107 | 0 | 2.4 × 103 | 1 | 2.4 × 103 |
| 144 | 7.5 × 107 | 0 | 4.4 × 104 | 3 × 103 | 8.7 × 104 |
| 172 | 5 × 107 | 0 | 1.5 × 106 | 4.6 × 104 | 2 × 107 |
a
293T cells were transfected with the full set of 12 expression plasmids (complete) or with all plasmids except the M-encoding helper plasmid (−T7/M). At different time points, the virus titer of the culture supernatants was determined.