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HA cleavage in the presence of plasminogen. MDBK cells infected with wild-type WSN or mutant WSN-453R or WSN-453L virus were incubated in medium containing 0.1% FCS, 5 μg of human plasminogen/ml, or 0.5 μg of TPCK-trypsin/ml. Viral proteins were labeled with [35S]Met-[35S]Cys (50 μCi/ml). After lysis of purified virions, HA proteins were immunoprecipitated with anti-HA monoclonal antibodies and separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
