Figure 2.

Identification of transcriptomic changes in specific T cell subsets of SAP by transcriptome analysis of scRNA-seq. (A) UMAP visualization of transcriptional heterogeneity in human T cells. T cells are further categorized into six subsets, with their respective names provided at the bottom of the graph. Different colors are employed to differentiate between each cluster, with dashed circles indicating the primary cell types. (B) Dot plot demonstrates the expression percentage and expression levels of DEGs across various T cell clusters. (C) Comparison of SAP dataset and healthy control dataset using UMAP embedded graphs. (D) Visualization of T cell clusters in SAP patients and healthy controls using the UMAP downscaling approach. (E, F) Main enriched GO and KEGG pathways for significant DEGs in the CD8⁺T_c01_GZMH cluster using cluster Profiler. (G) Volcano plots illustrate the DEGs identified in the CD8⁺T_c01_GZMH cluster, with upregulated genes marked in red and downregulated genes in blue. (H) Violin plot depicts the expression patterns of DEGs within the CD8⁺T_c01_GZMH cluster in SAP patients and healthy controls. Control, healthy controls; SAP, severe acute pancreatitis.