Figure 4.

Inference and analysis of cell-cell communication using CellChat. (A) The bar chart illustrates both the total (left) and interaction strength (right) of ligand-receptor interactions between distinct cell subpopulations in SAP patients and the control group. (B) The heatmap offers a more comprehensive representation of the quantity and intensity of interactions among diverse subpopulations. The colored bars at the top present the cumulative values for each column as indicated in the heatmap (incoming signals). The colored bars on the right depict the sum of values for each row (outgoing signals). In the color bars, red (or blue) signifies an augmentation (or reduction) in signals within the SAP dataset compared to the control dataset. (C) A comparative analysis of the intensity of inbound and outbound interactions within individual subpopulations between the SAP dataset and the control dataset is presented. The dot size reflects the volume of communication. (D) Evaluation of alterations in cell communication within two monocyte subpopulations featuring significantly elevated interaction strengths in the SAP dataset in contrast to the control dataset. (E, F) An assessment of considerably enhanced signals among diverse monocyte subpopulations and between monocyte subpopulations and DC cell subpopulations in the SAP dataset and the control dataset. Empty cells denote a zero probability of communication. Control: healthy controls, SAP: severe acute pancreatitis.