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. 2024 May 4;29(10):3195–3207. doi: 10.1038/s41380-024-02572-y

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Schema of chemogenetic modulation using DREADD. Injection of AAV-CaMKIIα-hM4Di-mCherry into the WT and Grin2b^C456Y/+ ACC was followed by locomotor and sensory tests with CNO/DMSO treatment. b–e Chemogenetic inhibition of ACC neurons does not alter the open-field locomotion and anxiety-like behavior (center time) of Grin2b^C456Y/+ mice (2–3 months) (n = 9 mice [WT-DMSO], 8 [WT-CNO], 10 [HT-DMSO], 9 [HT-CNO], three-way ANOVA [distance moved/center time], two-way ANOVA [total distance moved/center time]). f–i Chemogenetic inhibition of ACC neurons normalizes the sensory hypersensitivity of Grin2b^C456Y/+ mice (2–3 months) in the electronic von Frey, thermal place-preference, and electric foot-shock tests but not in the hot-plate test (n = 16 mice [WT-DMSO], 15 [WT-CNO], 15 [HT-DMSO], 15 [HT-CNO] for von Frey, 13, 13, 15, and 12 [thermal place preference], 15, 14, 16, and 14 [hot-plate], 12, 9, 9, and 9 [electric foot-shock], two-way ANOVA with Tukey’s test [von Frey, thermal place-preference], two-way ANOVA [hot-plate], two-way ANOVA with/without Tukey’s test [electric foot-shock; significant genotype x drug interaction for the 2nd but not 1st shock]). Significance is indicated as * (<0.05), ** (<0.01), *** (<0.001), or ns (not significant).