FIGURE 2.

Effect of the preventive protocol of treatment with GABA-Mo on visceral pain induced by DNBS in rats. Animal nocifensive response associated with visceral pain was assessed by measuring the extent of the abdominal withdrawal response (AWR) to colorectal distension (CRD; 0.5–3 mL; A-B). Animal visceral sensitivity was assessed by measuring the extent of the abdominal contraction (EMG amplitude) related to visceromotor response (VMR) to colorectal distension (CRD; 0.5–3 mL; C-D). The test was performed 7 days (A,C) and 14 days (B,D) after colitis induction. The test was performed 7 days (A) and 14 days (B) after colitis induction. *P < 0.05, **P < 0.01 and ***P < 0.001 vs Control + vehicle treated animals. ^∧ P < 0.05 vs DNBS+ vehicle treated animals.