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. 2024 Sep 20;15:1466824. doi: 10.3389/fphar.2024.1466824

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Copyright © 2024 Lucarini, Benvenuti, Di Salvo, D’Antongiovanni, Pellegrini, Valdiserra, Ciampi, Antonioli, Lambiase, Cancelli, Grosso, Di Cesare Mannelli, Bellini, Ghelardini and Fornai.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of DNBS treatment and preventive protocol with GABA-Mo on astrocytes and microglia activation in the spinal cord. The graphs show the quantification of GFAP immunoreactivity (A) and the density of GFAP-positive cells (B), with representative images showing the expression of GFAP (purple); (C), 14 days after DNBS injection. The figure reports the quantification of the percentage of reactive Iba-1-positive cells (D) and the density of Iba-1-positive cells (E), with representative images showing the expression of Iba-1 (green); (F), 14 days after DNBS injection. The quantitative analysis of immunofluorescence was performed by collecting independent fields from the dorsal horns of the spinal cord. *P < 0.05 vs. Control + vehicle group. ^∧ P < 0.05 vs. DNBS + vehicle group. Original magnification: ×20.