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. 2024 Oct 3;15:8578. doi: 10.1038/s41467-024-52379-2

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 8 — a The steatotic mouse models underwent intravenous injection of rmFGF21 (0.5 mg/kg) or tail vein injection of AAV8-hAAT-Fgf21 before I/R injury. b 15-HETE content in the livers from each group 6 h after reperfusion (n = 4, per group). c Serum ALT and AST 6 h after reperfusion (n = 5, per group). d H&E staining, ROS staining and TUNEL staining and quantification results of liver sections from each group 6 h after reperfusion (n = 5, per group). e Quantification assessment of infiltrating CD11b⁺ and MPO⁺ cells in the livers of each group (n = 5, per group). f The general schematic diagram. LT, liver transplantation; AA, arachidonic acid. I/R, ischemia/reperfusion; HFD, high-fat diet; ALT, alanine aminotransferase; AST, aspartate aminotransferase; HE, hematoxylin and eosin; ROS, reactive oxygen species; DHE, dihydroethidium; TUNEL, terminal deoxynucleotidyl transferase dUTP nick end labeling; MPO, cytosolic myeloperoxidase. Two-tailed t-test. Statistic data are presented as the mean ± SD, error bars represent the means of at least three independent experiments. p values are shown on the graphs, p < 0.05 was considered statistically significant. Source data are provided as a Source Data file. Figure 8a, f created with BioRender. com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license (https://creativecommons.org/licenses/by-nc-nd/4.0/deed.en).