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. 2024 Oct 3;14:23011. doi: 10.1038/s41598-024-73611-5

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 2 — Histology of gonophore in various developmental stages of Physalia utriculus. Sagittal section of a gonophore with a major axis diameter of 200 μm (A). Horizontal section of a gonophore with a major axis diameter of 200 μm (B). Sagittal section of a gonophore with a major axis diameter of 250 μm (C), 400 μm (D) and 800 μm (E). From left to right: brightfield, DAPI fluorescence image, phalloidin fluorescence image, and DAPI/phalloidin stacked image. DAPI signals targeting host nuclear DNA (blue), and fluorescent phalloidin signals targeting muscular actin filaments (red), respectively. Epithelial cells (Ec), gastrovascular cavity (Gca), gastrodermic cells (Gce) and putative germ cells (pGc) were seen in every developmental stages.