FIGURE 4.

Rab3-Myc is colocalized with TRPL after 4 h and 12 h of light adaption. (A) Analysis of the colocalization of TRPL and Rab3-Myc by immunohistochemistry in cross- and longitudinal-sections through ommatidia at different time points of orange light illumination. Flies were initially kept in the dark for 1 day and were then exposed to orange light for 15 min, 30 min, 45 min, 1 h, 2 h, 4 h, 8 h, and 12 h. TRPL was detected with an α-TRPL antibody and Rab3-Myc with an α-Myc antibody. Rhabdomeres were visualized with phalloidin and nuclei with DAPI. The images on the right illustrate an enlargement of the marked areas. Arrowheads indicate colocalization of TRPL with Rab3-Myc at time points 4 h and 12 h. Scale bar: 10 μm. (B) Quantification for colocalization of Rab3-Myc with TRPL after 15 min, 30 min, 45 min, 1 h, 2 h, 4 h, 8 h and 12 h of orange light illumination. The TRPL channel was chosen for the selection of the areas to be quantified. Colocalization was assessed using Pearson correlation. Error bars: SEM (n = 3).