FIGURE 7.

In RabX2 mutants TRPL is released from the ER after 15 min dark adaptation but does not reach the rhabdomere after 90 min. (A) Localization of TRPL in cross-sections of Ey-uS-Cas9/+ control and RabX2 CRISPR mutant ommatidia. After 16 h of orange light exposure, flies were dark-adapted for 15 min, 45 min, and 90 min. Sections were probed with α-Cnx99A and with α-TRPL antibodies. Rhabdomeres were stained with phalloidin and nuclei with DAPI. Images at the bottom show magnifications of the indicated areas above. Scale bar: 10 μm. (B) Colocalization of TRPL with the ER marker Cnx99A after 16 h of orange light illumination and 15 min of dark adaptation. Colocalization was assessed using Pearson correlation. The TRPL channel was chosen for the selection of the areas to be quantified. Statistically significant differences as analyzed by a two-way ANOVA calculation with Bonferroni correction are indicated (ns not significant) Error bars: SEM (n = 4–5).