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. 2024 Jul 22;20(10):1085–1108. doi: 10.1038/s44320-024-00052-7

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

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Figure EV2 — (A) Pair plot showing the CSC calculated in mosquito C6/36 cells using indicated transcription inhibitors. Lower triangle shows scatterplots of CSCs between inhibitors. Diagonal shows density plot of CSC for each inhibitor. Upper triangle shows Pearson correlation coefficient of CSCs between inhibitors. (B) Heatmap showing Spearman rank correlations between known CSCs in indicated species. P = 8.91e–05 for Human/Mosquito, P = 1.08e–05 for Human/Zebrafish, P = 4.13e–01 for Human/Frog, P = 0.343 for Human/Yeast, P = 7.94e–03 for Human/Fly, P = 3.25e–05 for Mosquito/Zebrafish, P = 9.29e–03 for Mosquito/Frog, P = 0.000181 for Mosquito/Yeast, P = 4.13e–14 for Mosquito/Fly, P = 8.70e–09 for Zebrafish/Frog, P = 0.0295 for Zebrafish/Yeast, P = 4.87e–06 for Zebrafish/Fly, P = 0.00562 for Frog/Yeast, P = 9.31e–06 for Frog/Fly, P = 1.97e–07 for Yeast/Fly. Color of the tile indicates correlation coefficient. *P < 0.05, **P < 0.01, ***P < 0.001. (C) 1nt frameshift reporters designed based on mosquito codon optimality were co-transfected into 293T human cells with a vector encoding for mCherry as an internal control. Bar plots showing that the 1nt frameshift reporter enriched in optimal codons displayed higher GFP/mCherry fluorescence intensity than its nonoptimal counterpart measured by flow cytometry analysis. Results are shown as the averages of GFP/mCherry fluorescence intensity ± standard error of the mean from two independent experiments with four biological replicates per experiment (P = 2.524e–8, unpaired t test).