Fig. 8. In vivo antitumor activity of cRGD- mix Man-pRNC_{Thioether+DEA}@R848 after combination with antibodies in mice with a large initial B16F10 tumor volume.

a Schematic illustration of the treatment timeline. b, c Average and individual tumor volume growth curves after different treatments (n = 7 mice per group). d Body weight changes of mice after treatments (n = 7 mice per group). e Survival curves of tumor-bearing mice post different treatments (n = 6 mice per group). f H&E Ki67 and TUNEL images of tumor slices. Scale bar = 50 μm. g, h Quantitative analysis of fluorescence intensity of Ki67 and TUNEL. Data are presented as mean ± SD (n = 3 independent experiments). Statistical significance was calculated through one-way ANOVA for multiple comparisons using a Tukey post-hoc test (b) or log-rank (Mantel-Cox) test (e).