TABLE 1.
Name | Sequence (5′–3′) | Location |
---|---|---|
PrimerE | ATGTCTGCAAACGGTTGCCCAA | ZtCpx1 |
PrimerF | CTACAACTTCGCGCTGCTGAC | ZtCpx1 |
PrimerG | ATGAAGGGTTGTCTCAATCATCTG | ZtCpx2 |
PrimerH | CTACTGGACATCGTTCTGAGGA | ZtCpx2 |
PrimerK | GTGCTCACCGCCTGGACGACTAAAC | Middle of hph gene |
PrimerL | GATGAGACCCGGCGACAAGT | Downstream of ZtCpx2 |
PrimerM | TTCGACCGTGGCTTGACACC | Upstream of ZtCpx1 |
PrimerN | TCCACCCAAGCGGCCGGA | Beginning of geneticin gene |
hph‐F | CTCGATGAGCTGATGCTTTG | hph gene |
hph‐R | GTCAATGACCGCTGTTATGC | hph gene |
ZtβTUB‐F | GCCCAGACAACTTCGTGTTC | β‐tubulin gene |
ZtβTUB‐R | ACGACATCGAGAACCTGGTC | β‐tubulin gene |
Q‐ZtCpx1‐F | ACAACGCCAATCTCGACAAG | ZtCpx1 |
Q‐ZtCpx1‐R | GACTCAATGGCGACATTTCC | ZtCpx1 |
Q‐ZtCpx2‐F | TCCTAAATCCGAGCCTTTCC | ZtCpx2 |
Q‐ZtCpx2‐R | TCAACCCACTGCCAAGAATC | ZtCpx2 |
Q‐ZtCat1‐F | GATGCACATGAAGAGCAAGC | ZtCat1 |
Q‐ZtCat1‐F | GAAACTCGCCCTTCTCAATG | ZtCat1 |
Q‐ZtCat2‐F | ACTTTGGCGTTCAGGTCATC | ZtCat2 |
Q‐ZtCat2‐F | ACTCCGACGTTCTGAATTGG | ZtCat2 |
SK‐Sep‐348 | ATCACCCTCGGCATGGACGAGCTCTACAAGATGTCTGCAAACGGTTGCCCAATC | ZtCpx1 |
SK‐Sep‐349 | CCACAAGATCCTGTCCTCGTCCGTCGTCGCCTACAACTTCGCGCTGCTGACC | ZtCpx1 |