Figure 6.

TET1 regulates mTOR activation in HASM cells. (A) Representative immunoblots illustrating the role of TET1 in mTOR activation. Nonasthmatic and asthmatic HASM cells were treated with 1 μm NSC-370284 (NSC) for 48 hours. They were then stimulated with PDGF (10 ng/ml, 10 min) or left unstimulated. mTOR activation was evaluated by immunoblot analysis. (B–E) Treatment with NSC attenuates the PDGF-induced phosphorylation of p70S6K, 4E-BP, S6, and Akt. Two-way ANOVA was used for statistical analysis. Data are mean ± SEM (n = 6 donors/group). *P < 0.05.