Figure 1.

Characterization of plasma extracellular vesicles (EVs). (A and B) NanoSight tracking analysis of EVs from infants on lower fraction of inspired oxygen (LO₂) (A) and EVs from infants on higher fraction of inspired oxygen (HO₂) (B). (C–E) Transmission EM of LO₂-EVs (C; red arrow) and HO₂-EVs (D; red arrow) showed that the HO₂-EVs were smaller in size (E). (F–H) Western blots demonstrated expression of EV markers CD9, CD63, and CD81 in the LO₂-EVs and the HO₂-EVs (F), with LO₂-EVs having higher expressions of CD63 (G) and CD 81 (H). Scale bars, 200 nm; n = 4 or 5 per group. *P < 0.05 and **P < 0.01.