Figure 3.

Tracking adoptively transferred EVs. ExoGlow-Vivo–labeled LO₂-EVs, HO₂-EVs, and PBS were injected into the retroorbital sinus of normal newborn mice at postnatal day 10. (A and C) The brains (A) and lungs (C) were dissected at 2 hours for ex vivo imaging. (B and D) Immunostaining for pan-EV markers CD9, CD63, and CD81 showed EVs (brown, red arrows) in the subgranular zone of the brain (B) and small vessels and alveolar septum of the lungs (D) in both LO₂-EV–injected and HO₂-EV–injected mice. Scale bars, 50 μm.