Minimal inhibitory concentrations of synthetic brevibacillin 1 and its analogues 7–20 against selected bacteria.

#	Peptide	Minimal inhibitory concentration (μg mL−1)
		Gram-positive					Gram-negative
		S.a a	L.m a	B.s a	E.f a	C.p b	P.a c	S.e c	E.c c	C.c d
1	Brevibacillin (Brevi)	2	2	1	4	4	1	64	32	8
7	Brevi Val4	4	1	2	16	4	2	>128	32	16
8	Brevi Val4,5	4	4	8	32	16	4	>128	64	32
9	Brevi Ile8	2	1	0.5	2	2	1	64	32	8
10	Brevi COOH	>128	128	>128	>128	>128	>128	>128	>128	32
11	Brevi CONH2	8	2	4	16	16	4	>128	>128	>128
12	Brevi Thr1	2	4	1	16	4	2	>128	32	16
13	Brevi Ser1	8	1	2	>128	8	4	>128	32	32
14	Brevi Ala1	2	1	2	4	2	1	>128	64	8
15	Brevi Abu1	1	0.5	1	2	2	1	>128	>128	4
16	Brevi Val1	1	1	1	4	4	1	>128	>128	8
17	Brevi Abu1/Val4,5	2	4	4	>128	8	2	>128	64	16
18	Brevi Abu1/CONH2	2	2	2	64	8	2	>128	>128	16
19	Brevi N-Ac-Ile/Abu1	2	2	4	32	2	2	>128	>128	16
20	Brevi N-Ac-Ile/Abu1/CONH2	>128	>128	>128	>128	>128	>128	>128	>128	>128

^aIncubated in TSB (tryptic soy broth) for 20 h at 37 °C.

^bIncubated in RCM (reinforced clostridial medium) for 20 h at 37 °C.

^cIncubated in LB (Luria–Bertani) for 20 h at 37 °C.

^dIncubated in Brucella for 48 h at 37 °C.