FIG. 3.

Endogenous RT activities of pNL4-3KFS wild-type (WT) and mutant virions. (A) ³²P-labeled viral DNA products made by wild-type and mutant virus particles. Incubation conditions and methods for the analysis of the DNA products by electrophoresis in a denaturing agarose gel are given in Materials and Methods. ³²P-labeled λ HindIII size markers are shown on the left. (B) PCR analysis of unlabeled DNA products made by mutant and wild-type virus particles. The DNA products were amplified using appropriate primer sets, as described in Materials and Methods. The negative control (Mock) is a reaction mixture containing the reaction buffer but not virus. One nanogram of pNL4-3KFS DNA (10, 13) was used as a positive control. The size of each PCR product is shown on the right; the specific product amplified is indicated on the left.