TABLE 1.

Phenotypes of HIV-1 wild-type and mutant virions

Virus	Virus production (%)a ± 1 SD	Infectivity by:		Presence of:
		Replication kineticsb	MAGIc (%)	Endogenous RTd	Viral DNA in cellse	Cone-shaped coref
WTg	100	+	100	+	+	+
W23A	71 ± 12	−	<0.5	+	−	−
F40A	59 ± 16	−	<0.5	+	−	−
D51A	99 ± 22	−	<0.5	+	−	−

^aVirus production was assayed as RT activity in the supernatant of transfected cells. Values were converted to percentages of wild-type level for each of nine independent transfections and were averaged. 

^bViral infectivity was assayed by measuring RT activity in the supernatant of infected CEM cells. 

^cInfectivity in the MAGI assay in a single round of infection. The numbers of blue cells were normalized to the RT activity of input virions and are reported as percentages of wild type. 

^dEndogenous RT activity was assayed as described in Materials and Methods. 

^eVirions obtained from transfected 293T cells were used to infect CD4-positive HeLa indicator cells, which were then lysed. Viral DNA was amplified by PCR. 

^fCone-shaped viral cores were detected by transmission electron microscopy in thin sections of cells producing virus. 

^gWT, wild type.