FIG. 1.

Diagram of the rBRSV genome, illustrating the construction of chimeric rBRSV. The G gene alone was replaced with an artificial gene encoding the BPIV-3 HN to create rBRSV-HN, the F gene alone was replaced with an artificial gene encoding the BPIV-3 F protein to create rBRSV-F, or both BRSV G and F were replaced with BPIV-3 HN and F (rBRSV-HNF). The location of the ORFs is shown as shaded (BRSV) or open (BPIV-3) rectangles. The glycoprotein genes are shown as enlargements in which gene start signals are represented by triangles and gene end/polyadenylation signals are shown as bars. The synthetic restriction sites used for cloning are indicated as arrowheads, with the location in the respective genome noted underneath. The genome length of the recombinant viruses is noted on the left.