FIG. 3.

Demonstration of viral transcripts by Northern hybridization. Total RNA of cells infected with the indicated viruses was isolated and separated by gel electrophoresis on a denaturing 1% agarose gel, blotted onto nitrocellulose, UV-cross-linked, and hybridized with [α-³²P]dCTP-labeled specific probes. (A) BRSV G-specific probe; (B) BRSV F-specific probe; (C) BPIV-3 HN-specific probe; (D) BPIV-3 F-specific probe. mRNA transcripts are indicated by arrowheads on the right.