Fig. 9.

Rho-kinase inhibitor Y27362 inhibits PAR₂-mediated sensitisation of TRPV4 by neutrophil elastase Oocytes were injected with cRNA coding for TRPV4 0.5 ng or TRPV4 0.5 ng/PAR₂ 10 ng and incubated for 2 days in ND96 solution. GSK1016790A-mediated whole-cell currents were measured using the two-electrode voltage-clamp technique. Oocytes expressing TRPV4 (white bars) were pre-incubated for 15 min in Ca²⁺-free solution in the absence (−) or in the presence (+) of Rho-kinase inhibitor Y27362 (10 μM). Oocytes expressing TRPV4/PAR₂ (black bars) were pre-incubated for 15 min in elastase (3 μM) or trypsin (8 nM) in the absence (−) or in the presence (+) of Rho-kinase inhibitor (10 μM). Columns represent mean ΔI_GSK1016790A values. N indicates the number of batches of oocytes. Numbers inside the columns indicate the number of individual oocytes measured. ***p<0.001, unpaired t test