FIG. 5.

Inability of P to facilitate nuclear localization of N NLS mutants. Wild-type GFP-N (A) or GFP-N NLS (B) mutant coexpressed with P. DAPI-stained cells are shown on the left. GFP epifluorescence micrographs are shown on the right, and overlays of these images are shown in the middle. Wild-type proteins accumulated in a subnuclear site (top row), and the NLS mutant combination accumulated in cytoplasmic aggregates (bottom row). The yeasts were grown and visualized as for Fig. 2. The red arrows indicate sites stained with DAPI, while GFP fluorescence is indicated by yellow arrows.