FIG. 6.

Deletion analysis of the P protein karyophillic domain. Full-length copies or portions of the P protein were cloned in frame with GFP and expressed in the presence of N or without the N protein. (A) Wild-type GFP-P had nuclear and cytoplasmic accumulation when expressed alone. (B) GFP-P coexpressed with N resulted in a complete shift of fluorescence to the nucleus. (C) GFP-P lacking the C terminus of P exhibited a shift from the cytoplasm to the nucleus. (D) GFP-P deletions lacking the N terminus of P, aa 40 to 124, accumulated in the cytoplasm. Coexpression of the N protein did not affect the localization of the GFP-P deletions (data not shown). The yeast cells were grown and visualized as described in the legend to Fig. 2.